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Mitochondrial oxygen consumption rate measurement BEAS-2B cells (CSE exposed and unexposed) were trypsinized and collected in RPMI-1640 cell culture medium, 100 l/well of the cell suspension at cell density 50,000 cells/well was seeded in duplicates in a Seahorse XF Pro96 microplate (Agilent Technologies, Santa Clara, CA, USA)

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The earthy burleys provide mild molasses and nuts with a very light smoky woodiness and even lighter cocoa hit

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