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3 Williamson LM, Hull D, Mehta R, Reeves WG, Robinson BH, Toghill PJ

The report, from the Truth Initiative, found that Netflix's 2016-17 programming showed 866 instances of tobacco use, compared to 299 instances in its 2015-16 programming

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In the second step, 7 l cDNA from step-1 was amplified using N gene forward primer (5-CACATTGGCACCCGCAATC-3), N gene reverse primer (5-GAGGAACGAGAAGAGGCTTG-3) and probe (5-FAM-ACTTCCTCAAGGAACAACATTGCCA-QSY-3) using Taqman mastermix (Thermo Fisher)

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