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Fasting and leptin modulate adipose and muscle uncoupling protein: divergent effects between messenger ribonucleic acid and protein expression

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N2a cells were transfected using Lipofectamine LTX and PLUS reagents (Invitrogen) with the following constructs: pAAVhSynmitoQC, mycParkin, pcDNA6.3 empty vector, EGFPParkin (human, gift from E

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