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Case studies and best practices: New Zealand recently passed a new law that stated that anyone born post 2008 would not be legally allowed to buy a packet of cigarettes or any tobacco products

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16S rRNA Amplicon Analysis The raw sequence data were subjected to ambiguity filtering and quality filtration using NGSQC toolkit (Patel and Jain, 2012), and the paired-end reads were assembled using FLASH (Mago and Salzberg, 2011)