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doi: 10.3389/fneur.2022.790884 68 LeeKAShinKSKimGYSongYCBaeEAKimIKet al

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RNA extraction, quantification of miRNA expression and qPCR Total RNA was extracted from the lung tissue samples of the screening cohort and from PBECs derived from non-COPD and COPD patients by TRIzol reagent method (Molecular Research Center, Cincinnati, OH) according to manufacturer's guidelines

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Circulation: Cardiovascular Quality and Outcomes , January 2017