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CSE was prepared fresh for each experiment by bubbling the smoke from one cigarette through 10 ml of 5 mM Tris-HCl buffer (pH 7.4) in a 15-ml conical tube for 2.5 min

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According to federal regulations, open dating is not required except on infant formula (Use-By dates are mandated for these products)

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If the purchaser of any lot fails to comply with any such requirement the auctioneer may put up again and re-sell the lot