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Using puffing regimens modelled on puffing topography data from 19 experienced e-cigarette users who switched between 18 and 6 mg/mL e-liquids with and without power adjustments, differences in daily exposure to carbonyl compounds and estimated changes in cancer risk were assessed by production of aerosols generated using a smoking machine and analyzed using gas and liquid chromatography

Cotinine is an active metabolite of nicotine that remains in the blood for 1820 hours, making it easier to analyze due to its longer half-life

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The mitochondrial gene expression in CSE-unexposed cells showed no change in gene expression of MT-CYB and MT-RNR1 during the 25 weeks culture, but MT-CO2 expression gradually increased over time (Fig