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Trypsin in 50 mM TEAB pH 8.5 was added to the samples to a final protease-to-protein ratio of 1:20 (w/w) and incubated for 24 h at 37 C

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As per the application for a government-issued headstone, Castles military service was listed as the 1 st Company, 5 th Brigade of the Enlisted Ordnance Corps

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