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Billerica, MA, USA) without anesthesia for 5 min per scan

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1.1 placebo Moderate-to-severe depression (PHQ-9 10): remission 52% vs

ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, Minneapolis, MN, USA) connected to a Waverunner LT342 digital oscilloscope (LeCroy, Chestnut Ridge, NY, USA) before procedure, after ligation and therapy application, and before sacrifice

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