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It also plays an important role in maintaining the intracellular free CoA pool, when excess intra-mitochondrial acyl- groups are present, through the reverse transport of fatty acyl groups as acylcarnitines by the L-carnitine system (Figure 1

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The cells were then incubated with antibodies against arrestin3 or HA (1:1000 dilution) for 1 h at 20 C, followed by washing with PBS and incubation with the secondary antibodies (Alexa 555-conjugated antibodies at 1:500 dilution)

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